Added replicate analysis to evaluate Buscar on- and off-scores across plates#89
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axiomcura wants to merge 6 commits intoWayScience:mainfrom
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Added replicate analysis to evaluate Buscar on- and off-scores across plates#89axiomcura wants to merge 6 commits intoWayScience:mainfrom
axiomcura wants to merge 6 commits intoWayScience:mainfrom
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axiomcura
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This PR introduces a replicate analysis for compounds, where each cell type includes four technical replicates.
The goal is to evaluate how Buscar computes on- and off-scores when replicates are distributed across different plates.
We define:
Paired replicates: comparisons between identical compounds across replicates (e.g., compound X vs. X2, X3, X4)
Non-paired replicates (negative control): comparisons between different compounds (e.g., compound X vs. A, N, Z)
This setup allows us to assess whether Buscar can distinguish true replicate consistency from unrelated compound variation.
Related issue: #73