diff --git a/.changeset/calm-cats-guard.md b/.changeset/calm-cats-guard.md
new file mode 100644
index 000000000..6e749b024
--- /dev/null
+++ b/.changeset/calm-cats-guard.md
@@ -0,0 +1,5 @@
+---
+'@callstack/repack': patch
+---
+
+Prevent guarded require initialization from throwing in strict-mode bundles by safely copying runtime property descriptors.
diff --git a/packages/repack/src/plugins/RepackTargetPlugin/implementation/__tests__/guardedRequire.test.ts b/packages/repack/src/plugins/RepackTargetPlugin/implementation/__tests__/guardedRequire.test.ts
new file mode 100644
index 000000000..f73dc9ee7
--- /dev/null
+++ b/packages/repack/src/plugins/RepackTargetPlugin/implementation/__tests__/guardedRequire.test.ts
@@ -0,0 +1,44 @@
+import vm from 'node:vm';
+
+const guardedRequireImplementation = require('../guardedRequire');
+
+describe('guardedRequire implementation', () => {
+  it('preserves runtime properties in strict mode', () => {
+    // Reproduce the strict wrapper emitted when an Rspack runtime is built as
+    // ESM. The previous direct assignments threw while copying Function
+    // intrinsics before React Native could execute the application entry.
+    const result = vm.runInNewContext(`
+      (function () {
+        'use strict';
+        var self = {};
+        var factories = { page: function () { return 'page'; } };
+        var __webpack_require__ = function originalWebpackRequire(moduleId) {
+          return moduleId;
+        };
+        Object.defineProperty(__webpack_require__, 'm', {
+          configurable: true,
+          enumerable: false,
+          get: function () { return factories; }
+        });
+        __webpack_require__.federation = { name: 'remote' };
+
+        (${guardedRequireImplementation
+          .toString()
+          .replaceAll('$globalObject$', 'self')})();
+
+        return {
+          value: __webpack_require__('page'),
+          factories: __webpack_require__.m,
+          federation: __webpack_require__.federation,
+          descriptor: Object.getOwnPropertyDescriptor(__webpack_require__, 'm')
+        };
+      })()
+    `);
+
+    expect(result.value).toBe('page');
+    expect(result.factories.page()).toBe('page');
+    expect(result.federation.name).toBe('remote');
+    expect(result.descriptor.enumerable).toBe(false);
+    expect(typeof result.descriptor.get).toBe('function');
+  });
+});
diff --git a/packages/repack/src/plugins/RepackTargetPlugin/implementation/guardedRequire.ts b/packages/repack/src/plugins/RepackTargetPlugin/implementation/guardedRequire.ts
index dd7834586..00a05cebe 100644
--- a/packages/repack/src/plugins/RepackTargetPlugin/implementation/guardedRequire.ts
+++ b/packages/repack/src/plugins/RepackTargetPlugin/implementation/guardedRequire.ts
@@ -27,10 +27,29 @@ module.exports = function () {
     }
   }
 
-  // Copy all properties from the original function to the wrapped function
+  // Copy the original require's runtime properties without invoking getters.
+  // Function intrinsics such as `prototype`, `arguments`, and `caller` are
+  // non-configurable on the wrapper and cannot be redefined. Assigning every
+  // property directly is also unsafe in strict-mode bundles because some
+  // function properties are read-only and would throw during startup.
   Object.getOwnPropertyNames(originalWebpackRequire).forEach((key) => {
-    // @ts-ignore
-    guardedWebpackRequire[key] = originalWebpackRequire[key];
+    const sourceDescriptor = Object.getOwnPropertyDescriptor(
+      originalWebpackRequire,
+      key
+    );
+    const targetDescriptor = Object.getOwnPropertyDescriptor(
+      guardedWebpackRequire,
+      key
+    );
+
+    if (
+      !sourceDescriptor ||
+      (targetDescriptor && !targetDescriptor.configurable)
+    ) {
+      return;
+    }
+
+    Object.defineProperty(guardedWebpackRequire, key, sourceDescriptor);
   });
 
   // @ts-ignore