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@@ -31,6 +31,7 @@ FCS data. The code needed to run the template is in the newest release of [TASBE
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* Multiple bead files can be compared by listing out their sample names in the "Sample Name" cell within the "Rainbow Beads" section. (i.e. Beads,Beads2,Beads3). The "Bead Comparison Tolerance" determines how identical you want the bead files to be. The results of the comparisons will be included in the TASBESession log located at the bottom of the sheet.
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* The "Constitutive/Input/Output" cells within the "Fluorochromes" section are optional for batch analysis but required for plusminus and transfer curve analysis. If this feature will be used, exactly one channel must be marked as Constitutive. The minimum is 2 colors with the second color acting as both the input and output. (It only needs to be labeled as one of the two.) For 3 or more colors, all pairwise combinations between labeled inputs and outputs will be analyzed. Colors not labeled will be ignored.
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* The "Relevant Channels" cell within the "Color Translation" section determines which colors to consider when creating the Color Model. A value of "all" means all listed colors will be used.
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* Size beads feature can be used by filling out the "Size Beads" and "Non-fluorescence Channels (unprocessed)" sections.
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### Comparative Analysis
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* "Comparison Group(s)" contains sample column name-value pairs that determine which samples in the "Samples" sheet are compared. The format is "Sample Column Name = Value". Multiple name-value pairs can be listed out to further specify the comparison groups (i.e. ColName1 = Value1, ColName2 = Value2). Leave this section blank if all samples should be considered for analysis.
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