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qcAndArchR.yml
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35 lines (28 loc) · 1.15 KB
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## *** QC-FILTERING PARAMS ***:
# Minimum number of unique reads passing cells must have
minUniqueReads: 100
# Proportion of of total reads expected to be background. (Between 0 and 1)
backgroundRatio: 0.03
# Top Percent of Cells which will be used to calculate QC metric thresholds (from 0-100)
topPercentCells: 10
## *** ARCHR ANALYSIS PARAMS ***:
## .ARROW CREATION PARAMS
# Minimum TSS enrichment score (as defined by ArchR) passing cells must have
minimumTSS: 1
# Minimum Fragments
minimumFragments: 0
## DIMENSIONALITY REDUCTION PARAMS
# Number of iterations of LSI to be run during dimensionality reduction (on TileMatrix)
LSI_Iterations: 2
## CLUSTERING PARAMS
# Maximum number of clusters to group cells into
maxClusters: 2
# Number of neighboring sample points used for UMAP.
# Larger values result in more global views while smaller values result in more local data
# being preserved from (2-100)
nNeighbors: 50
# Specifying whether to run certain aspects of automated analysis
# Generate UMAP from dimensionality reduction output?
plotUMAP: true
# Calculate marker genes from ArchR extrapolated genescores per cluster?
getMarkerGenes: false