Describe the Problem
DN4_fuGFP sequence on Benchling does not have the correct overhangs
https://benchling.com/openbioeconomy/f/lib_XnxELeu3-reporter-genes/seq_VgVROBPJ-dn4_fugfp/edit
Sequence Data
5' overhang is GTCA, should be AGGT
3' overhang is CGAA, should be AAGC
It seems to be a duplicate of N2N3_fuGFP
Follow-Up Actions
Trace sequence back through spreadsheet and check sequencing data to establish if this is a duplication on Benchling or the synthesis was incorrect https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit?gid=807017147#gid=807017147
Describe the Problem
DN4_fuGFP sequence on Benchling does not have the correct overhangs
https://benchling.com/openbioeconomy/f/lib_XnxELeu3-reporter-genes/seq_VgVROBPJ-dn4_fugfp/edit
Sequence Data
5' overhang is GTCA, should be AGGT
3' overhang is CGAA, should be AAGC
It seems to be a duplicate of N2N3_fuGFP
Follow-Up Actions
Trace sequence back through spreadsheet and check sequencing data to establish if this is a duplication on Benchling or the synthesis was incorrect https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit?gid=807017147#gid=807017147