Is your feature request related to a problem? Please describe.
We have seen issues with toxicity in pTi expression vectors with slightly toxic genes stored in cloning strains (i.e. no T7 RNA Pol available). pOpen_v3 has also had issues reported for toxic parts (see this thread on the forum).
Describe the solution you'd like
Analyse plasmids using : https://www.denovodna.com/software/predict_promoter_calculator
might need to create an account to see/use it but that is free) based on this paper: https://www.nature.com/articles/s41467-022-32829-5
and/or CryptKeeper
https://www.biorxiv.org/content/10.1101/2024.09.05.611466v1.full
Is your feature request related to a problem? Please describe.
We have seen issues with toxicity in pTi expression vectors with slightly toxic genes stored in cloning strains (i.e. no T7 RNA Pol available). pOpen_v3 has also had issues reported for toxic parts (see this thread on the forum).
Describe the solution you'd like
Analyse plasmids using : https://www.denovodna.com/software/predict_promoter_calculator
might need to create an account to see/use it but that is free) based on this paper: https://www.nature.com/articles/s41467-022-32829-5
and/or CryptKeeper
https://www.biorxiv.org/content/10.1101/2024.09.05.611466v1.full