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README.md

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This repository contains code to reproduce analyses presented in the paper 'Identification of methylation-sensitive human transcription factors using meSMiLE-seq'.
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This repository contains code to reproduce analyses presented in the paper 'Identification of methylation-sensitive human transcription factors using meSMiLE-seq'. The 'main' branch contains analyses requested during the revisions, the 'initial_release' branch is the archive of the 1st version.
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> [!NOTE]
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> **Prerequisites**
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- analysis of classical SMiLE-seq experiments by using a Fisher's exact test to prefilter raw sequencing reads before *de novo* motif discovery via ProBound
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2. **meSMiLEseq**
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- analysis of methylation-sensitive SMiLE-seq data to create methylation-aware binding models and *k*-mer scatterplots
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- analysis of methylation-sensitive SMiLE-seq data to create methylation-aware binding models and *k*-mer scatterplots, as well as separated ProBound analyses for methylated and unmethylated libraries
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3. **WGBS_analysis**
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- exemplary analysis pipeline using PRDM13 to show CG methylation patterns at individual motif occurrences in cells by intersecting ChIP-seq and WGBS data

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